Monday, January 14, 2008
Preparation of Competent E.Coli Cells
It is necessary to make the E.coli cells competent for the transformation. Ecoli M229 strin was made competent to DNA via CaCl2 treatment. Transferred 1.5ml of log phase culture to a microfuge tube and kept it at 0C for 10min. Above solution was centrifuged at 4000rpm for 10min and the supernatant was remo
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